T4 ligase promega

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August undefined, 2024

WebT4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl 2, 100mM DTT and 10mM ATP. WebSep 12, 2016 · The internal T7 promoter of pCR XL TOPO was removed using deletion PCR followed by T4 DNA ligase-T4 polynucleotide kinase-DpnI (NEB, USA) treatment prior to transformation into XL10-GOLD ultracompetent cells (Agilent Technologies, USA). The resulting pT7-EV71 infectious clone was subjected to full-genome sequencing. ...

T4 DNA Ligase Product Information Sheet 9PIM180 - Ohio …

WebDec 1, 2024 · 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/Hind III fragments at 16°C in 20 minutes. Promega 2800 Woods Hollow Rd. Madison, Wisconsin United States Phone: 800-356-9526 Fax: 800-356-1970 Company Profile Website: http://www.promega.com Citations (15) WebT4DNA Ligaseとユニークな2X Rapid Ligation Bufferを組合せています。連結されたDNAはトランスフォーメーションをする前に精製する必要がありません。また、特製の2X Rapid Ligation Bufferは使用前にATPやMg2+を加える必要はありません。迅速: 突出（粘着）末端なら5分間、平滑末端なら15分間効率的: 他社製品に較べ優れたライゲーション効率便 … thinktrio scam alert

ResDE-Dependent Regulation of Enterotoxin Gene Expression in …

WebT4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. WebDescription. The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf (+) Vector with Eco R V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid. 2X Rapid Ligation Buffer allows reactions to be completed in one hour ... WebT4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The … thinktube

Fusarium wilt of cyclamen : early detection and biocontrol

T4 DNA Ligase from Promega Biocompare.com

Web1.5ml Designed for use with T4 DNA Ligase to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent … WebApr 24, 2009 · Restriction endonucleases and T4 DNA ligase were obtained from Promega. Genomic DNA of B. cereus was purified as described in reference 16. Plasmid DNA was purified using anion-exchange columns (Promega). PCR amplification of DNA was carried out with Taq polymerase (Roche Molecular Biochemicals). thinktvWebT4 DNA Ligase Product Information Sheet 9PIM180. Product Contents Quality Control Assays. Promega Corporation. 2800 Woods Hollow Road Madison, WI 53711-5399 USA … thinkture property

"WebPlease Enquire. T4 DNA Ligase. 1–3u/µl/100u (Weiss units) ¥ 9,000. T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´ … " - T4 ligase promega

T4 ligase promega

Web• 1 μL of T4 DNA ligase (Promega) • x μL water up to 20 μL total. *BsaI-HF is optimized for different buffer conditions and doesn't work well ** Promega T4 Ligase buffer has been shown to offer the highest activity for BsaI enzyme. Other T4 ligase enzymes will work, but Promega enzyme with Promega buffer is highest efficiency. WebT4 RNA Ligase 一本鎖RNAまたは一本鎖DNAの5'-末端のリン酸基と3'-末端の水酸基をATP依存的に結合 M1051 関連製品 T4 Polynucleotide Kinase DNAシークエンシング …

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WebT4 DNA Ligase, Blue/White Cloning Qualified Protocol Instructions for Use of Product (s) M1801, M1804, M1794 Literature # 9PIM180 T4 DNA Ligase catalyzes the joining of two … WebDNA Ligase Master Mixes are pre-mixed, ready-to-use formulations that are specially formulated for Blunt/TA or Sticky Ends. The tool NEBCloner can help you select the best ligase formulation for your needs. The following tips will help to achieve maximum results from your ligation reactions. Reaction Buffers. T4 DNA Ligase Buffer should be ...

WebLa fusariose vasculaire du cyclamen est une maladie causée par le champignon tellurique Fusarium oxysporum f. sp. cyclaminis. Elle est considérée comme l’une des maladies les plus graves du cyclamen et se traduit par des pertes … WebApr 13, 2024 · The resulting monophosphate RNA is intramolecularly circularized using T4 RNA ligase to produce a circular RNA with the 5′ untranslated regions (UTR) fused to the 3′ end polyA tail followed by ...

WebDec 1, 2024 · 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/Hind III fragments at 16°C in 20 minutes. Promega 2800 Woods Hollow Rd. Madison, Wisconsin United States Phone: 800-356-9526 Fax: 800-356-1970 Company Profile Website: http://www.promega.com Citations (64) WebDNA Ligase Selection Chart While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. To select a ligase based upon the type of substrate present, please visit Substrate-based Ligase Selection Chart.

WebBy product: T4 DNA ligase, T4 RNA ligase, E. coli DNA ligase, Pfu DNA ligase, Quick ligase, Tth DNA ligase, others ... Promega Corporation, Qiagen N.V., Thermo Fisher Scientific, Inc., among others. In this report, key players and their strategies are thoroughly analyzed to understand the competitive outlook of the market.

Web小鼠精胺氧化酶基因SMO启动子的结构和功能研究.pdf thinktuningWebT4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation and cloning. thinktv membershipWebT4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5′-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant Escherichia coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. Revised 4/18. Complete Protocol PDF (113k) thinktronicWebT4 DNA ligase is an enzyme that fixes broken DNA and seals it – similar to super glue. This particular DNA ligase was isolated from bacteriophage T4. During DNA replication or recombination, a break or a ‘nick’ in the backbone of DNA frequently occurs. thinktv clearanceWeb2X Rapid Ligation Buffer. 1.5ml. Designed for use with T4 DNA Ligase to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. Ligate DNA inserts into plasmid vectors in as little as 15 minutes. No purification necessary prior to transformation of ... thinktpmsWebSupplier: Promega The LigaFast™ rapid DNA ligation system is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors. Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. Ligation of cohesive ends in five minutes, blunt ends in 15 minutes at room temperature thinktv 14WebPromega Corporation · 2800 Woods Hollow Road·Madison, WI 53711-5399 U.S.A. ·Toll Free in the USA 800-356-9526 ·Telephone 608-274-4330 ·Internet www.promega.com Usage Information I. Description T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a … thinktube.com