Phosphinothricin basta
WebDescription DL-Phosphinothricin is a herbicide that has been used as a selection agent for the genes bar or pat and is the active ingredient in Basta®, Glufosinate Ammonium, PPT, … WebBasta is a nonselective foliar applied herbicide for the control of undesirable mono- and dicotyledonous plants in orchards, vineyards, and plantations for minimum tillage, and as …
Phosphinothricin basta
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WebSep 1, 2016 · Increasing the transformation efficiency is essential and is being carried out using biolistics, Agrobacterium and microinjection of protoplasts methods and selection …
WebConventional selection for BASTA resistance (conferred by bar, the phosphinothricin N-acetlytransferase gene) (D'Halluin et al. 1992) involves spraying the aerial parts of germinated seedlings and can be performed in non-sterile conditions with seeds sown directly on soil at relatively high densities. WebMar 30, 2015 · We use Glufosinate-ammonium (also known as phosphinothricin or BASTA) purchased from Sigma at a concentration of 10 ug/mL plus 150 ug/ml timentin (Ticarcillin …
WebProduct Specifications Phosphinothricin Glufosinate or Glufosinate ammonium Also known as Bayer’s Basta TM Formula: C 5 H 15 N 2 O 4 P MW: 198.16 g/mol Storage/Handling: … WebMar 3, 2015 · The T-DNA from these vectors confers phosphinothricin (Basta) resistance to plants. Gateway-compatible destination vectors for tissue-specific expression After creating pMCS:GW and pMCS:YFP-GW, we cloned the presumptive promoters of several genes with previously described expression patterns (Table 1 ) into these vectors to create a set of ...
WebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. 1 publication Catalytic activity acetyl-CoA + phosphinothricin = CoA + H+ + N-acetylphosphinothricin
WebJan 12, 2024 · Herbicides remain the primary tool for implementing weed management to maintain high yields of economically important crops. Phosphinothricin (PPT), also known as glufosinate (commonly used in the form of glufosinate-ammonium), is the major active ingredient in many non-selective herbicide formulations, including BASTA ® (BASF SE, … citb online servicesWebSynthetic phosphinothricin (glufosinate) is a component of two top-selling herbicides (Basta and Liberty), and is widely used with resistant transgenic crops including corn, cotton and canola. Recent genetic and biochemical studies showed that during phosphinothricin tripeptide biosynthesis 2-hydroxyethylphosphonate (HEP) is converted to ... citb online practice testWebBialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase. The bar gene product was purified and shown to be a modifying enzyme which acetylates phosphinothricin or demethylphosphinothricin but not bialaphos or glutamate. The bar gene was subcloned and its nucleotide sequence was determined. diane box wormanWebThe Seekers - Massachusetts (2002) diane bowyer milford miWebNov 26, 2024 · 24 hygromycin B, phosphinothricin (BASTA) and nourseothricin resistance genes, meaning that 25 positive transformants can be identified from non-sterile seeds in as little as four days after 26 stratification and transferred to soil before the onset of visible microbial contamination. 27 Conclusion citb online test practiceWebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl-(methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am … diane bower halifax paWebJun 9, 2024 · Basta herbicide resistance assay For Basta herbicide resistance assays, seeds were sown as above and 10 to 14 day old wildtype or Basta resistant seedlings grown in 2.5 inch pots were treated with 25 ml of 3 mg/l Basta, results were collected 7 days after exposure. Agrobacterium mediated leaf-infiltration for transient gene expression diane bourgeois baton rouge general