WebVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. WebOct 18, 2024 · The purified PCR product was cloned using NheI and EcoRI enzymes into the pET28b vector with an N-terminal His 6-tag and thrombin site. The construct was then transformed into BL21(DE3) E. coli strain (Novagen). The cells were cultured in LB media containing 30 µg/mL of kanamycin at 310 K until an OD 600 of 0.6. The temperature was …
Novagen® pET-28a(+) DNA, MilliporeSigma VWR
WebAug 16, 2011 · At a Glance pET-28a-c (+), image provided by Novagen Notes The pET-28a (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique … http://wolfson.huji.ac.il/purification/PDF/Protease_fusion_cleavage/NOVAGEN_Thrombin_kit.pdf bourbon street webcam cat\u0027s meow
pET-28b(+) Sequence and Map - SnapGene
WebThe Novagen pET-28a-c (+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. pET-28a (+) DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS 分析证书 用户协议 Citations Vector Map Vector Sequence … WebMilliporeSigma™ Novagen™, Biotinylated Thrombin. Removes enzyme from cleavage reactions using immobilized streptavidin. Manufacturer: MilliporeSigma™ 696723. … WebNovagen’s Biotinylated Thrombin and Restriction Grade Thrombin are functionally tested for specific cleavage of the sequence Le uValProArg ↓GlySer in recombinant fusion proteins. … guild acoustic with case