Chk1 s345
WebSpecificity Specific for Chk1 (phospho S345) in ELISA, with <1% reactivity with the non-modified form of the immunogen. Tested applications Suitable for: WB, IHC-P, ELISA … WebWe propose that Atr is a major regulator of Chk1 in response to DNA damage based on the following observations: (1) Chk1 phosphorylation on S345 is increased in response to …
Chk1 s345
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WebCHK1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). CHK1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression. Further, CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that … WebThe primary antibody against S345 of Chk1 was purchased from Cell Signaling Technology (Danvers, MA, USA); Chk1 and β-actin antibodies were purchased from Santa Cruz Biotechnology Inc (Dallas, TX, USA). The protein expression signal on blots was quantified by Fujifilm Multi Gauge software (Tokyo, Japan). The ratio of Chk1-S345 to Chk1 ...
WebIn siGFP-transfected cells, p-Chk1 (S345) and p- Cdk2 (T14, Y15) were detected, indicating that HU treatment induced cell cycle arrest ( Figure 4C). p-Chk1 and p-Cdk2 levels were lower in HU ... WebChk1 Monoclonal Antibody (T.59.2), Invitrogen™-Mouse Monoclonal Antibody Compre Chk1 Monoclonal Antibody (T.59.2), Invitrogen™ en Fishersci.es. Español English; Cambiar país. Solicite una oferta › Centro de Ayuda y Soporte › Español English; Cambiar país; 0. No offer available ...
WebDec 19, 2011 · Notably, the dissociation of Chk1 from Chk1-S in DDR appeared dependent on Chk1 phosphorylation at S345/S317, because the Chk1(S345A/S317A) mutant did not dissociate from Chk1-S during camptothecin treatment (Fig. 3E). In DDR, Chk1 activation depends on ATR-mediated phosphorylation (7, 13, 14). WebJun 5, 2015 · Levels of phosphorylated CHK1 (S317 and S345) following UV treatment of TRE-BCBL-1-RTA cells treated with doxycycline for varying lengths of time. At each time point following the addition of doxycycline, cells were treated with 20 Jm − 2 UV and levels of phosphorylated CHK1 were assessed by western blot.
Phospho-Chk1 (Ser345) Antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345. This antibody does not cross-react with Chk1 when phosphorylated at other sites. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification
phonetically pronounce wordsWebJun 10, 2014 · Chk1 inhibition by V158411 in the leukemia and lymphoma cell lines induced DNA fragmentation and cell death that was both caspase dependent and independent, and prevented cells undergoing mitosis. ... was obtained from Millipore; Chk1, pChk1 (S317), pChk1 (S345), Cdc2, pCdc2 (Y15), Cyclin B1 and pH2AX (S139) from Cell Signaling … phonetically pronouncedWebJun 21, 2004 · Chk1 P-S345 and Chk2 P-T68 antibodies were obtained from Cell Signaling Technology (Beverly, MA). P-S1981 ATM antibody was from Rockland (Gilbertsville, PA). In Vitro Kinase Assays. Kinase assays were performed with Flag-tagged recombinant ATM or ATR proteins produced in human embryonic kidney (HEK) 293 cells as described in ref. 28. phonetically respelledWebMay 24, 2024 · Hello, I Really need some help. Posted about my SAB listing a few weeks ago about not showing up in search only when you entered the exact name. I pretty … how do you test for crohn\u0027sWebJun 4, 2024 · Phosphorylation of the effector kinase CHK1 at S345 and S317 by ATR enhances its kinase activity, resulting in dissociation from chromatin, phosphorylation of CDC25A-C phosphatases, which prevent the progression of the cell cycle [ 11, 12, 13 ]. phonetically regularWebWe show that in human cells, Chk1 is phosphorylated on serine 345 (S345) in response to UV, IR, and hydroxyurea (HU). Overexpression of wild-type Atr enhances, whereas overexpression of the kinase-defective mutant Atr inhibits S345 phosphorylation of Chk1 induced by UV treatment. how do you test for cteWebHuman Phospho-Chk1 (S345) Antibody Summary. Detects human, mouse, and rat Chk1 when phosphorylated at S345. This antibody does not recognize Chk1 when … phonetically regular definition